Plant Tissue Culture in the Classroom and Home
for the Serious Hobbyist, Gardener, Teacher, Student or
Nurseryman
Plant tissue culture involves the sterile
growth of plants in bottles for the purpose of mass production.
Through the use of plant hormones and other
growth regulators, small plant parts can be induced to produce hundreds of small
"plantlets" which can be further developed and grown in greenhouses or as house
plants.
Using a microwave oven or a pressure cooker,
supplies found in your kitchen, plus the contents of the "Kitchen Culture Kit©,
you can mass propagate hundreds of your favorite plants in your kitchen or classroom.
In this workshop you will make your own
media, disinfect and culture plant leaves, axillary buds and seeds, and discuss trouble
shooting and internet resources. These techniques can then be applied to other species.
| Time |
Tentative Schedule (Photos) for Beginner
Class |
| 9:00 |
Introductions
Fill out information sheets; hand out class schedule
Discuss what everyone wants to accomplish
Safety and food; location of aprons, gloves, goggles, etc. |
| 9:20 |
Presentation
on plant tissue culture in the home and classroom (part 1) |
| |
Break |
| 10:20 |
Media
preparation using a microwave: demo by instructor followed by media preparation by
students (break into groups) |
| 11:20 |
Discuss
clean boxes and aseptic technique
Assemble PVC boxes and prepare areas |
| 12 Noon |
Lunch
(provided) and discussion |
| 1:00 |
Demo
of disinfection and culture of: leaf, axillary bud, seeds |
| 1:15 |
Students disinfect and culture:
--African violet leaves
--Axillary buds
--Seed or orchid pod (if available)
--Other? |
| |
Break
(as it fits your individual schedule) |
| 4:00 |
Discuss
problems, trouble shooting, alternative protocols, internet connection and webpage
resources;
Share experiences with "seasoned tissue culturists" at meeting |
| 5:00 |
Workshop
officially ends/discussions continue as needed |
| |
|
|
